CASP5 anticorps

N° du produit ABIN319133

L’anticorps Lapin Polyclonal anti-CASP5 a été validé pour WB et IF. Il convient pour détecter CASP5 dans des échantillons de Humain.

Aperçu rapide pour CASP5 anticorps (ABIN319133)

Antigène: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CASP5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-CASP5 anticorps

Specificité: This antibody recognises an epitope located within the internal region of human Caspase-5, a 47 kDa cysteine protease, otherwise known as ICH-3.

Purification: Antisera to human Caspase-5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogène: A 14 amino acid peptide from the centre of human Caspase-5.

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 1.0g/mL - 2.0g/mL, detects a band of approximately 48 kDa in Ramos celllysates. (Predicted precursor MWT 47.8 kDa)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CASP5

Antigène: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Autre désignation: Caspase-5

Sujet: Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes. Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta. The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (1).Synonyms: CASP-5, CASP5, ICE(rel)-III, ICH-3 protease, ICH3, TY protease

ID gène: 838

NCBI Accession: NP_001129581

UniProt: P51878

Pathways: Inflammasome